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The detection of serum progesterone levels is primarily accomplished through blood tests, which is currently one of the most commonly used and reliable methods

Jul 15, 2024

The detection of serum progesterone levels is primarily accomplished through blood tests, which is currently one of the most commonly used and reliable methods

Below are the specific steps and precautions for serum progesterone level detection:

I. Preparation Before Testing

Time Selection:

  • For pregnant women, it is generally recommended to test progesterone levels weekly from the onset of threatened abortion symptoms until week 12 of pregnancy or during termination of pregnancy. For non-pregnant women, testing can be scheduled as needed, such as on days 22-24 of the menstrual cycle, to evaluate luteal function.
  • Note that the timing of testing should be in accordance with medical advice to ensure the accuracy of the results.

Dietary Considerations:

  • Prior to testing, it is essential to maintain a fasted state to minimize the impact of food on the test results. Pregnant women should particularly avoid breakfast before blood draw.

Emotional Stability:

  • Maintaining emotional stability before testing is crucial to prevent factors such as stress and mood fluctuations from interfering with the results.

II. Testing Procedure

Blood Draw:

  • The healthcare professional will tie a tourniquet around the arm of the pregnant woman or individual requiring the test, instructing them to clench their fist to engorge the veins. The practitioner will then locate a well-vascularized vein, disinfect the area, and insert a puncture needle at an angle into the vein, advancing parallel to the vessel to a suitable depth to collect enough blood for analysis.
  • Typically, 3-5ml of blood is drawn to meet the testing requirements.

Sample Processing:

  • The blood sample must be processed promptly. This involves centrifuging the blood to separate the serum, which is then stored under appropriate temperature conditions for later testing. For instance, the serum can be frozen at -30°C.

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