Dog pregnancy detection reagents are primarily tools used to determine whether a canine is pregnant. Common forms include test strips and detection cards.

Here's a detailed introduction to these reagents:

I. Primary Principle

Dog pregnancy detection reagents primarily rely on measuring the levels of a specific hormone or substance within the canine's body to determine pregnancy status. Among these, Relaxin is a commonly used marker. It is a polypeptide hormone synthesized and secreted by the corpus luteum and placenta during pregnancy. Its levels gradually increase with the progression of pregnancy and rapidly decrease after parturition. Therefore, by detecting the level of Relaxin in a dog's body, one can preliminarily determine if it is pregnant.

II. Product Types

• Test Strips: There are various branded dog pregnancy test strips available on the market, such as Elide and Mengge. These strips are often user-friendly and moderately priced, making them popular among pet owners. The strips detect pregnancy by collecting urine or blood samples from the dog and following the instructions on the package. The color change or line display on the strip indicates pregnancy status.
• Detection Cards: In addition to test strips, detection cards are also available. These cards are typically more sensitive and accurate but require some level of operational skill. They determine pregnancy by measuring the level of Relaxin in the dog's whole blood or serum. Blood or serum samples from the dog need to be collected and processed according to the instructions on the package.

III. Usage Method

• Sample Collection: Collect urine, blood, or serum samples from the dog as per the instructions on the package. For test strips, urine samples are commonly used, while for detection cards, blood or serum samples are required.
• Operation Steps: Follow the instructions on the package carefully. Typically, you will need to apply the sample to the sample well on the strip or card and wait for a few minutes (usually ranging from a few to several dozen minutes) for a color change or line display to occur.
• Result Interpretation: Use the interpretation criteria provided on the package to determine pregnancy status. Generally, if a specific color change or line display occurs (e.g., both lines turning red), it indicates that the dog is pregnant. If only one line appears or no line is visible, it suggests that the dog may not be pregnant or further testing is required.

IV. Precautions

• Operational Norms: Always follow the instructions on the package precisely to ensure accurate results.
• Sample Freshness: Ensure that samples are tested promptly to prevent changes in hormone or substance concentrations over time.
• Results as Reference: Test results should be considered as references only and not definitive diagnoses. If pregnancy is suspected, consult clinical symptoms or other routine tests for confirmation.
• Storage Conditions: Store detection reagents in a dry, cool, and light-shielded place to prevent moisture or degradation from affecting the test outcomes.